Product Name: Trypsin
Chemical Name: Trypsin
Synonyms: Protease, EC 3.4.21.4
CAS Number: 9002-07-7
Recommended Use: Protein digestion in laboratory, cell culture applications, food industry
Supplier Identification: Supplier contact details listed on package label, including phone for emergencies
Classification: Respiratory sensitizer, Eye irritant, Skin irritant
Label Elements: Warning, Exclamation mark symbol
Hazard Statements: Causes serious eye irritation, May cause respiratory irritation, May cause an allergic skin reaction
Precautionary Statements: Avoid breathing dust, Wash hands thoroughly after handling, Use only in well-ventilated area, Wear protective gloves, eye protection
Route of Exposure: Inhalation, skin, eyes
Chemical Name: Trypsin
Concentration: 90-100% (depending on grade)
Impurities: May contain traces of other proteases used in enzyme preparations
Molecular Formula: C41H67N11O5
Molecular Weight: Approximately 23.3 kDa (for the enzyme)
Inhalation: Remove victim to fresh air, monitor for breathing difficulty, seek medical attention if symptoms continue
Skin Contact: Remove contaminated clothing, wash skin thoroughly with soap and water, consult a doctor if irritation persists
Eye Contact: Flush eyes with water for at least 15 minutes, hold eyelids apart, obtain medical advice
Ingestion: Rinse mouth with water, do not induce vomiting, never give anything by mouth to an unconscious person, get medical help
Note for Medical Staff: Treat symptomatically and monitor for allergic responses
Suitable Extinguishing Media: Water spray, foam, carbon dioxide, dry chemical
Unsuitable Media: Direct water jet
Special Hazards: Burns to proteinaceous material may produce harmful decomposition products including nitrogen oxides and carbon monoxide
Protective Equipment: Firefighters should wear full protective clothing and self-contained breathing apparatus
Advice: Evacuate area, avoid breathing fumes
Personal Precautions: Avoid breathing dust, wear appropriate personal protective equipment, keep unprotected persons away
Environmental Precautions: Prevent spillage from entering drains, water courses
Cleanup Methods: Sweep up material without creating dust, place in sealed bag or container for proper disposal, ventilate affected area, clean surfaces after material pickup
Decontamination: Wash with plenty of water and mild detergent
Handling: Minimize dust generation, avoid contact with skin, eyes, and clothing, use in well-ventilated area, follow good industrial hygiene practices
Storage Conditions: Store at 2–8°C, keep container tightly closed, protect from moisture, keep away from incompatible materials like strong oxidizers
Storage Incompatibility: Strong acids, bases, oxidizing agents
Engineering Controls: Use laboratory fume hood or local exhaust system to limit exposure to dust or aerosols
Personal Protection: Wear protective lab coat, gloves, safety glasses or goggles, if dust is present use NIOSH-approved respiratory protection
Hygiene Measures: Wash hands after handling, avoid eating or drinking in work area, remove contaminated clothing and wash before reuse
Exposure Limits: No specific exposure limits established for trypsin, control dust levels to lowest practicable concentrations
Appearance: White to off-white powder or crystalline solid
Odor: None
pH: 6.5–8.5 (in solution)
Melting Point: Not determined (decomposes upon heating)
Solubility: Freely soluble in water
Boiling Point: Not applicable
Partition Coefficient: Not available
Density: Not available
Vapor Pressure: Not applicable
Autoignition Temperature: Not available
Chemical Stability: Stable under recommended storage conditions, degrades in presence of moisture, heat
Reactivity: Reacts with oxidizers
Conditions to Avoid: Moisture, heat, open flames
Hazardous Decomposition Products: Nitrogen oxides, carbon oxides may form during combustion
Polymerization: Will not occur
Acute Toxicity: Data not fully available for humans, occupational exposure may produce mild to moderate irritation of skin, eyes, and respiratory tract
Chronic Effects: Prolonged or repeated exposure can cause allergic sensitization by inhalation or skin contact
Inhalation: Sensitization may occur in some individuals
Skin Contact: Irritation, possible allergic dermatitis
Eye Contact: May cause significant irritation and redness
Ingestion: May cause mild gastrointestinal disturbances in large amounts
Carcinogenicity: Not listed as a carcinogen by NTP, IARC or OSHA
Ecotoxicity: Not anticipated to be harmful to aquatic life based on protein nature, but large quantities may affect water oxygen levels
Persistence and Degradability: Readily biodegradable in the environment
Bioaccumulation Potential: Low, enzyme degrades to amino acids
Mobility in Soil: Expected to have low mobility, binds to soil and organic matter
Other Adverse Effects: No specific data found
Disposal Method: Dispose of in accordance with local, regional, national, and international regulations, incinerate or landfill through approved chemical waste disposal contractor
Special Precautions: Prevent dust formation, properly label waste containers, do not allow to enter surface waters or drains
Contaminated Packaging: Empty containers should be rinsed and disposed of as chemical waste
UN Number: Not regulated
Class: Not regulated as dangerous goods
Packing Group: Not regulated
Special Precautions: Avoid temperature extremes, use sealed containers during transit
Transport Label: None required under normal conditions
OSHA Status: Not hazardous according to OSHA Hazard Communication Standard
TSCA Status: Listed
SARA Title III: Not subject to reporting under Sections 302, 304, 311, 312, or 313
California Proposition 65: Contains no chemicals known to the State of California to cause cancer, birth defects, or reproductive harm
Other Regulatory Listings: Compliant with applicable national and international regulations for laboratory, research, and industrial use
