Chemical Name: 2-[Bis(2-Hydroxyethyl)Amino]ethanesulphonic acid
Other Names: BES, N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid
CAS Number: 10191-18-1
Molecular Formula: C6H15NO5S
Recommended Uses: Common buffer for biochemical and biological research, pH control in electrophoresis, reaction media in protein purification
Supplier: Refer to specific product packaging for supplier identification and emergency contact details
Emergency Phone: Refer to product label or supplier documentation for a 24-hour emergency contact
Classification: Not classified as hazardous according to OSHA Hazard Communication Standard (29 CFR 1910.1200), GHS not deemed necessary for most laboratory settings
Main Hazards: Potential for mild irritation to eyes, skin, and respiratory tract from dust or prolonged exposure
Health Hazards: Short contact with powder can result in eye or skin discomfort, inhalation may irritate mucous membranes, ingestion can cause gastrointestinal upset
Environmental Hazards: Product is not considered a marine pollutant, not prone to bioaccumulation in aquatic environments, not persistent
Signal Word: None required
Pictograms: No pictogram required
Precautionary Statements: Avoid generating dust, use in well-ventilated areas, avoid unnecessary contact, store away from incompatible materials
Chemical Name: 2-[Bis(2-Hydroxyethyl)Amino]ethanesulphonic acid
Common Name: BES
Concentration: ≥99% purity
Impurities: Trace synthesis byproducts less than 1%
CAS Registry Number: 10191-18-1
EC Number: 233-469-7
Inhalation: Remove the person to fresh air, provide warmth and rest, seek medical assistance if coughing or breathing difficulty occurs
Skin Contact: Immediately wash skin with plenty of water and mild soap, remove contaminated clothing, consult medical support if irritation persists
Eye Contact: Rinse eyes cautiously with water for several minutes, remove contact lenses if present, continue rinsing, receive medical attention in the event of lasting discomfort
Ingestion: Rinse mouth with water, do not induce vomiting, give water if fully conscious, seek professional medical advice
Acute and Delayed Symptoms: Potential irritation to eyes, skin, and respiratory tract, no widespread reports of chronic effects from low-level exposure
Suitable Extinguishing Media: Use water spray, dry powder, carbon dioxide, or foam for surrounding fire
Extinguishing Media Not Recommended: Strong jets of water can spread product dust
Hazardous Combustion Products: Burning can release sulfur oxides, nitrogen oxides, carbon dioxide, carbon monoxide
Firefighting Equipment: Wear self-contained breathing apparatus, protective clothing
Unusual Fire/Explosion Hazards: Not readily combustible but generates irritating fumes during decomposition
Personal Precautions: Wear adequate protective gear to prevent eye and skin contact, minimize dust generation
Environmental Precautions: Prevent entry into drains, sewers, or water bodies
Clean-Up Methods: Sweep up spilled powder carefully, place in suitable container for disposal, ventilate spill area, clean residues with water and detergent
Handling Spills: Do not use tools that generate sparks or static electricity, avoid sweeping dust into the air
Handling Guidelines: Use gloves, lab coats, and goggles, minimize dust formation, work under local exhaust
Safe Storage Conditions: Store in tightly closed containers, dry, cool, and well-ventilated location, keep away from strong oxidizers and acids
Incompatibilities: Strong oxidizing agents, strong acids
Hygiene Practices: Wash hands after handling, avoid eating or drinking in the area, change contaminated clothing after working with the compound
Occupational Exposure Limits: No specific limits set by OSHA, ACGIH, or NIOSH
Engineering Controls: Operate in fume hood or with adequate general and local exhaust
Personal Protective Equipment: Safety goggles, chemical-resistant gloves (such as nitrile), lab coat, dust mask or respirator if airborne levels exceed comfort
Eye/Face Protection: Use chemical splash goggles
Skin Protection: Wear standard laboratory protective clothing
Respiratory Protection: NIOSH-approved particulate respirators required for excessive dust
Appearance: White crystalline powder
Odor: Odorless
pH (10 g/L): 5.8 - 7.2
Melting Point: 295–298 °C (with decomposition)
Boiling Point: Not applicable (decomposes before boiling)
Solubility: Soluble in water (approx. 70 g/L at 25°C), practically insoluble in organic solvents
Vapor Pressure: Not relevant at room temperature
Relative Density: 1.16 g/cm³
Partition Coefficient (log Kow): -3.5 (estimated, not bioaccumulative)
Explosive Properties: Not explosive
Oxidizing Properties: Not an oxidizer
Chemical Stability: Stable under recommended storage and normal laboratory conditions
Reactivity: Product remains inert under ordinary conditions, no violent polymerization
Incompatible Materials: Strong oxidizers, strong mineral acids can cause exothermic reactions
Hazardous Decomposition: Degradation yields sulfur oxides, nitrogen oxides, carbon monoxide, carbon dioxide at high temperatures
Conditions to Avoid: Prolonged heat, moisture, direct sunlight, and storage near reactive chemicals
Polymerization Risk: Not known to polymerize dangerously
Acute Toxicity: Oral LD50 in rats estimated above 2,000 mg/kg (low toxicity)
Skin Corrosion/Irritation: Mild irritation possible, not classified as a corrosive
Serious Eye Damage/Irritation: Dust may irritate if direct contact occurs
Respiratory/Skin Sensitization: No evidence of sensitizing properties
Mutagenicity: No mutagenic effects observed in standard tests
Carcinogenicity: Not listed as a carcinogen by IARC, NTP, or OSHA
Reproductive Toxicity: No specific studies reported reproductive harm
Other Effects: Overexposure to dust could increase respiratory discomfort
Ecotoxicity: Low potential for bioaccumulation, rapid breakdown under environmental conditions
Aquatic Toxicity: LC50 (fish, 96 h): >10,000 mg/L (practically non-toxic)
Persistence and Degradability: Readily biodegradable in water and soil, does not persist or accumulate
Mobility in Soil: High mobility in water, expected to move through soil and dilute rapidly
Bioaccumulation Potential: Very low (log Kow indicates unlikely to bioaccumulate)
Environmental Fate: No known adverse impact on aquatic or terrestrial organisms at laboratory release quantities
Waste Disposal Methods: Dispose of unused product, contaminated packaging, and cleaning materials in accordance with local, state, and federal regulations
Container Disposal: Empty containers should be triple-rinsed before disposal, punctured to prevent reuse
Special Precautions: Do not discharge to sewer or environment, handle all residues as chemical waste
Recycling Information: Not suitable for re-use due to contamination risk, avoid home or commercial compost
UN/NA Number: Not regulated as a dangerous good
DOT (Land): Not subject to DOT restrictions
IMDG (Sea): Not classified as a marine pollutant, not a regulated substance for shipping
IATA (Air): Not regulated as hazardous for air transport
Labeling Requirements: No hazard label required
Special Precautions: Secure packaging to prevent release of powder, no additional marking needed for international transport under current guidelines
U.S. Federal Regulations: Not subject to SARA Title III, CERCLA, or RCRA reporting requirements
TSCA Inventory: Listed on the TSCA inventory
Canada DSL/NDSL: Listed on the Domestic Substances List
EU REACH Registration: Registered under REACH with appropriate safety documentation
OSHA: Not classified as hazardous, general laboratory safety measures apply
Other Regulatory References: Not cited under California Proposition 65, not listed as carcinogenic or as a hazardous air pollutant
