Product Name: 2-[4-(2-Hydroxyethyl)-1-Piperazinyl]ethanesulfonic Acid
Chemical Formula: C8H18N2O4S
Synonyms: HEPES
CAS Number: 7365-45-9
Intended Use: Laboratory buffer in biochemical and biological research.
Manufacturer: [Insert actual manufacturer details]
Emergency Phone: [Insert actual emergency contact number]
Date Prepared: [Insert latest revision date]
GHS Classification: Not classified as hazardous under GHS.
Signal Word: None required
Hazard Statements: Dust or powder may cause eye and respiratory irritation. Not considered to present a significant hazard in normal academic or laboratory use.
Precautionary Statements: Avoid breathing dust. Use only with adequate ventilation. Wash hands thoroughly after handling.
Potential Health Effects: Inhalation may lead to respiratory tract irritation, coughing, or sneezing. Skin contact could lead to mild irritation, particularly in sensitive individuals. Eye contact typically causes discomfort, redness, or watering. Ingestion is not expected to cause acute toxicity, though gastrointestinal upset may occur in large doses.
Chronic Effects: Current evidence does not support long-term hazards in general laboratory contexts.
Chemical Identity: 2-[4-(2-Hydroxyethyl)-1-Piperazinyl]ethanesulfonic Acid
Purity: >99%
Other Components: No other ingredients contribute to chemical or physical hazards in this product; trace impurities are typical of analytical-grade chemicals but do not influence hazard classification.
Molecular Weight: 238.3 g/mol
General Advice: Move affected person to fresh air and keep at rest.
Eye Contact: Flush immediately with plenty of water for at least 15 minutes, lifting upper and lower eyelids occasionally. Seek medical attention if irritation persists.
Skin Contact: Remove contaminated clothing and wash skin thoroughly with soap and water. Seek medical advice if irritation continues.
Inhalation: Remove from exposure to fresh air immediately. Support breathing as required. Obtain medical attention if respiratory symptoms or cough develop.
Ingestion: Rinse mouth with water. Drink water to dilute. Do not induce vomiting. If the person feels unwell, seek medical advice.
Suitable Extinguishing Media: Use water spray, dry chemical, alcohol-resistant foam, or carbon dioxide. Select agent appropriate for surrounding fire.
Specific Hazards: Product itself does not support combustion. Combustion yields carbon oxides, nitrogen oxides, and sulfur oxides.
Protective Equipment: Firefighters should wear self-contained breathing apparatus and full protective gear.
Advice: Move containers from fire area if safe. Avoid inhaling fumes, smoke, or gases generated by fire.
Personal Precautions: Avoid breathing dust. Use suitable personal protective equipment including gloves, laboratory coat, and safety goggles.
Environmental Precautions: Prevent discharge to drains and surface water. Contain spill to smallest possible area.
Clean-Up Methods: Scoop up spilled solids and place in suitable waste containers. Wipe area with damp cloth to remove residue. Ventilate area and wash affected surfaces after removal.
Disposal: See disposal considerations below for correct disposal protocols.
Reporting: Report significant releases to local authorities as required by law.
Handling: Work in a well-ventilated area. Keep container tightly closed when not in use. Avoid inhalation of dust or contact with skin and eyes. Practice good personal hygiene after handling.
Storage: Store at room temperature, away from heat, moisture, and incompatible substances. Keep out of direct sunlight and ensure containers are tightly sealed.
Incompatibilities: Avoid storing with strong oxidizers or acids that could cause hazardous reactions.
Specific Practices: Prevent static discharge by grounding equipment. Use non-sparking tools during transfer.
Special Requirements: Limit access to trained personnel in laboratory settings.
Engineering Controls: Use local exhaust or general dilution ventilation to keep dust concentrations low. Safety showers and eyewash stations should be easily accessible.
Respiratory Protection: Wear a particulate-filtering mask if dust is generated.
Hand Protection: Chemical-resistant gloves, such as nitrile or latex.
Eye Protection: Safety goggles with side shields to prevent accidental splashes or dust exposure.
Skin Protection: Lab coats and clothing to minimize skin contact.
Exposure Limit Values: No established occupational exposure limits found for this chemical.
Hygiene Measures: Wash hands after use and before eating or drinking. Remove contaminated clothing before leaving work area.
Appearance: White crystalline powder
Odor: Odorless
Odor Threshold: No data available
pH: 5.0–6.0 (50 g/L in water at 25°C)
Melting Point: 234–238°C (decomposition may begin before full melting)
Boiling Point: Not applicable/decomposes
Flash Point: No data available (non-flammable)
Evaporation Rate: Not volatile
Flammability: Does not sustain combustion
Upper/Lower Explosion Limits: Not applicable
Vapor Pressure: Negligible at ambient temperature
Vapor Density: Not available
Specific Gravity: 1.49 (water=1)
Solubility: Very soluble in water
Partition Coefficient (n-octanol/water): Not determined
Auto-ignition Temperature: Not self-igniting
Decomposition Temperature: Above 200°C
Viscosity: Not relevant for solid
Chemical Stability: Stable under normal conditions of use, transport, and storage.
Reactivity: Non-reactive under standard laboratory conditions.
Possible Hazardous Reactions: Reacts with strong oxidizing agents, acids, or bases to produce irritating or toxic gases.
Conditions to Avoid: Exposure to extreme temperatures, moisture, or strong oxidants.
Hazardous Decomposition Products: Carbon dioxide, carbon monoxide, nitrogen oxides, sulfur oxides may form at elevated temperatures.
Polymerization: No dangerous polymerization expected.
Acute Toxicity: Low toxicity if inhaled, ingested, or touched. Rat oral LD50 > 5,000 mg/kg.
Skin Corrosion/Irritation: May cause mild irritation upon prolonged or repeated contact.
Eye Damage/Irritation: Dust particles can cause local irritation but are unlikely to cause serious eye injury.
Respiratory or Skin Sensitization: Not classified as a sensitizer.
Germ Cell Mutagenicity: No data indicating mutagenic effects.
Carcinogenicity: Not listed by IARC, NTP, OSHA, or ACGIH.
Reproductive Toxicity: Not expected to affect fertility or the developing fetus.
Target Organ Effects: No reports of specific target organ toxicity from available data.
Chronic Toxicity: Chronic exposure unlikely to result in adverse health effects under normal laboratory conditions.
Ecotoxicity: Not expected to be harmful to aquatic life at environmentally relevant concentrations. Lack of acute toxicity data signals minimal hazard.
Persistence and Degradability: Product is expected to degrade slowly in the environment through natural processes.
Bioaccumulative Potential: Low potential for bioaccumulation in aquatic organisms due to low log Kow.
Mobility in Soil: High solubility in water suggests rapid movement through soil and ground water.
Other Effects: No impact on ozone layer, global warming potential unstudied at present.
Avoid Release: Do not allow larger quantities to reach sewage system or water bodies.
Waste Disposal Methods: Dispose of this chemical as non-hazardous laboratory waste, in accordance with local, regional, and national regulations. Avoid flushing large quantities into drains.
Container Disposal: Rinse containers before discarding. Follow standard protocols for plastic, glass, or metal containers.
Recommended Treatment: Incineration or landfill in permitted waste treatment facility under government guidelines.
Other Considerations: Always consult relevant regulations for waste classification, as status can change over time.
UN Number: Not regulated as a dangerous good.
Proper Shipping Name: 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid (HEPES)
Transport Hazard Class: None
Packing Group: None
Marine Pollutant: No
Special Transportation Notes: Handle containers securely during all modes of transport to prevent formation of dust and inadvertent spills.
Labelling: No additional labelling requirements for ground, air, or sea freight under current regulations.
International Inventories: Product listed on TSCA (US), DSL (Canada), EINECS/ELINCS (EU), ENCS (Japan), and AICS (Australia).
OSHA Status: Not classified as hazardous under OSHA Hazard Communication Standard.
SARA Title III: Not listed.
California Proposition 65: Not listed as a chemical known to cause cancer, birth defects, or other reproductive harm.
WHMIS (Canada): Classified as non-hazardous.
EU Classification: Not classified as hazardous or dangerous under current legislation.
Other Information: Users and employers must abide by relevant national and local chemical safety regulations, particularly for workplace safety and environmental handling.
